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It was dissolved in DMSO (20 mmol/L) and diluted with cell growth medium for experimental usage.
Appropriate dilutions were made into culture medium for experimental analyses.
Wells containing PSPs were harvested, frozen and expanded in drug-free medium for experimental studies.
Upon arrival, we placed the hepatocytes in the incubator for 3 hr to equilibrate and then exchanged the shipping medium for experimental dosing media.
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After traumatising the slices, the medium was exchanged for experimental medium containing 4.5 μM PI.
The medium for the experimental and control group was added into the lower compartment of the Transwell chambers (500 μL/well).
The medium for the experimental and control group was added into the lower compartment of the Transwell chamber (500 μL/wel)l.
Hovagimyan doesn't have a distinct medium, but is recognized for experimental projects using hypertext, digital performance art, interactive installations, and HD video.
For experimental analysis, the medium was changed to experimental medium, which is oestrogen depleted, (phenol-red-free RPMI containing 5% charcoal-stripped, steroid-depleted FCS, glutamine and antibiotics as above) for 24 hours before undertaking the relevant assays as described below.
Cell lines were cultured in experimental medium for 48 hours before the initiation of experiments.
After pre-incubation, the culture medium was changed to experimental medium for 24 h: H2O2 (Thermo Fisher, 200 nM), Staur (Sigma, 1 nM).
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