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One of the most powerful and important stories through the medium for at least the last two years is representation.
Microcarriers were equilibrated in the culture medium for at least 30 min prior to the addition of the cells in order to maximize cell attachment.
The membranes fabricated with Nafion® and silicotungstic acid with and without thiophene still exhibited good mechanical strength and stability after they had been dipped in an acid or a basic medium for at least 10 months.
This suggests that XEN cells are stable in standard medium for at least short-term culture.
Transfected Leishmania strains were maintained in liquid medium for at least 24 h before plating on a drug-containing semi-solid medium.
To determine the stability of pigment expression cultures were transferred to fresh medium for at least five times in the dark.
Interestingly, we could maintain hNSCs as neurospheres in FPN medium for at least 5 subculturing passages, achieving a moderate but still important expansion of the cell population (10-fold versus 100-fold expansion in FPN- versus EF hNSCs).
After incubation in SPM medium for at least three hours, capacitation status was monitored by the detection of phosphotyrosines using rabbit anti-human phosphotyrosine polyclonal antibody (Zymed, South San Francisco, CA, USA).
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However, the medium for fermentation at optimum process variables contained 50 g/l glycerol.
Embryos were embedded in OCT medium for cryosection at 15 µm [41].
The plates were washed 4X with PBS and blocked with 10% RPMI medium for 2hrs at 37°C.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com