Sentence examples for medium for additional from inspiring English sources

The cells were then continuously cultured in EBM2 medium for additional 10 days (a total 14 days culture).

Cells were then switched to low-glucose growth medium (DMEM containing 1 g/l glucose plus 10% FBS) or maintained in regular growth medium for additional 48 h.

On the consecutive day, the old medium was discarded and the petri dish was preserved in a horizontal position with 10-mL medium for additional 24 h.

On the consecutive day, the petri dish was preserved in a horizontal position and the cells were cultured with 10 mL of fresh medium for additional 24 h (b).

The cells were incubated in DMEM with FBS and PSN at 37°C in a humidified 5%CO2/95%% air environment for 48 h, and after which were incubated with DNA-lipid mixture for 3 h, then the cells were washed twice and incubated in fresh medium for additional 5 h.

They were then transferred to the drug-containing medium for additional one or two days.

As shown in Fig.  2a, yeast cells were first cultivated in FAP-containing medium till stationary phase, and then an aliquot of the culture was transferred to fresh FAP-containing medium twice for additional rounds of growth.

For treating with RNA and protein de novo biosynthesis inhibitors, cells were pretreated for 1 h with Act D and CHX prior to the addition of DHT in the same medium for an additional 24 h.

Following 2 hr of drug exposure, cells were washed, and incubated in drug-free medium for an additional period of 2 hr.

After this period of pre-treatment, cells were switched to either high K+- containing differentiation medium, normal differentiation medium, or control medium for one additional week before harvesting cells for analysis.

After the initial 8 days of cortical development, structurally stable hESC cerebral organoids were incubated with 100 nM PD173074 or in drug-free medium for an additional 10 days, during the ongoing CZ expansion.