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Three miRNAs were selected for further studies, miR-487b, miR-410, with the highest fold change, and miR-409-3p miR-409-3p miR-409-3phange.
Moreover, 10 genes with lower or medium fold change around both INF-γ and TNF-α network were also analyzed using samples generated from infected animals.
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The changes in polysome distributions were hard to differentiate in the low-fold and medium-fold change sample RP mRNAs suggesting that the polysome gradient may have limited sensitivity in these ranges.
For osteogenesis assays, absorbance in experimental wells was normalized by dividing by the absorbance measured in untreated osteogenic cultures and reported as fold change from medium.
Absorbance or luminescence in experimental wells was normalized by dividing by the absorbance or luminescence measured in untreated cultures, and is reported as "fold change from medium".
All other samples were indicated as fold change compared with 0 h or medium control, respectively.
The size of the gene names represents the fold change (small = 1.5- to 10-fold, medium = 10- to 500-fold, large = 500 + fold expression).
Results were normalized to GAPDH and expressed as the fold change compared with samples from cells incubated in medium.
Data are plotted as fold change in LU when compared to LU generated in control medium treatments.
Only 6 hr of cultivation of bystander hESC in medium conditioned for 1 hr following irradiation of hESC with 0.2 Gy revealed modest (1.5– fold change) increase in the apoptotic cell death compared to sham-exposed cells (Fig. 7, E).
Table 3 shows the fold change of EMCP metabolites when increasing to ten times the cobalt concentration in the medium.
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