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Optimal matrix coating and adaption of continuous medium flow were elaborated for hepatocytes[56 59].
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Medium flow was generated by a magnetic stirrer in this system.
Furthermore, contrast medium flow is a dynamic process.
The medium flow was set at 0.2 mL min−1 [ 23].
Clamp C. I was opened and the medium flow was restarted.
The medium flow was then stopped on the pump and clamps C. I and C. III were closed and clamp C. II opened.
For the incubation at t = 308 days, the medium flow was stopped and nitrite was added to a final concentration of 1.3 mM in the bioreactor.
When the nitrite concentration was <0.1 or >1 mM, the medium flow was adjusted (influent varied between 0.3 and 1 l day−1).
The 100-mL holding volume of ODM with 60 g/L xylose and 20 g/L ethanol was inoculated to A620 0.5, and the population was allowed to grow batch-wise to stationary phase; then the feed medium flow was started.
Given that medium flow is essential for nutrient and metabolic exchanges, the WA properties of a scaffold are another important feature for developing a suitable scaffold for bone regeneration [ 47].
Reactors were inoculated directly through the rubber seal (25% of reactor volume) with fridge-stored stock cultures of Clostridium thermocellum, while medium flow was halted for 2 hours to allow cell acclimation.
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