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The cells are seeded on laminin (BD, Le Pont De Claix, France) coated cell culture inserts (pore diameter 3.0 μm, 0.33 cm; Falcon, BD, Le Pont De Claix, France) that are flipped over and placed in a medium flooded 12-well plate.
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Homes continue to be built in areas of high and medium flood risk.
The Environment Agency (EA) has issued a medium flood warning for areas including the market town of Langport.
The agency has warned of "significant disruption" on Friday and has issued a medium flood risk warning in Somerset over the next three days.
Regime B includes flood events with a medium duration (274 min), medium flood crest (0.206 m s−1), and minor runoff depth (0.7 mm).
Regime C includes flood events with the longest duration (822 min), medium flood crest (0.236 m s−1), and medium runoff depth (1.7 mm).
Small and medium floods including the once annual spring flood-pulse have been completely eliminated.
This aside, enhanced P uptake by rice plants in low-to-medium-P flooded soil has recently been reported by overexpression of a P transporter OsPht1 8 (OsPT8) (Jia et al., 2011; Wu et al., 2011).
To exclude that release of intracellular PLDs from dead or dying cells causes the observed activity, V8 medium of a flooded plate was carefully removed, fresh medium was added and after overnight incubation the extracellular medium was again tested for PLD activity.
Using a sterile cork borer of diameter 8 mm, 5 wells were dug into the Mueller Hinton agar medium which was initially flooded with 1 ml of the standardized inoculum of each test organism and the excess was drained using a Finn pipette.
After 1 h, the sample was flooded with serum-free medium (plating medium without FBS) and cultured for 5 7 days, or as indicated elsewhere.
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