We identified 533 genes significantly up-regulated under silicon starvation compared to subsequent time points after addition of silicate to the medium (Additional file 3; Additional file 9).
Proportionally, CK- N-glucosides accumulated strongly in all tested variants (149.01 – 254.81 pmol/g FW), with iP7G as the predominant metabolite regardless of growth medium (Additional file 2 and Additional file 3).
The wild-type strain of Synechocystis PCC 6803, (SD100) has recently been genetically modified using two distinct strategies designed to release free fatty acids (FFAs) directly into the cultured medium (Additional file 1: Figure S1, Additional file 2: Table S1) [ 17, 35, 37].
Also, PHB-producing cells showed similar growth between Nile red containing (0, 0.8, 2 µg/mL) and non-containing PHB-producing medium (Additional file 1: Fig. S3-B).
Consistent to this observation, MoS2-PEG-PEI kept their consistent hydrodynamic sizes at about 50 nm in the serum-containing cell medium (Additional file 1: Figure S1b).
After overnight growth, the mutants were transferred on Q-Tray bioassay plates containing LB solid medium (Additional file 1: Fig. S1b, gridding) and activity was detected after Congo Red staining.
Furthermore, the LipidGreen1 was not harmful for the bacterial growth on agar plates (Fig. 1) and in liquid medium (Additional file 1: Fig. S3), and thus could be used from the beginning of the bacterial culture.
Chlamydomonas reinhardtii was cultured in TAPx4 medium (Additional file 1: Table S1, supporting information) at 20 °C in an incubator (Infors, Switzerland) equipped with a shaker continuously rotating at 100 rpm and an illumination regime of 114.2 μmol phot m−2 s−1.
The mesocotyl length of indica accessions ranged from 0 mm to 46.0 mm, whereas that of japonica cultivars ranged from 0 mm to 16.4 mm on agar medium (Additional file 1: Table S1).
Although some of soluble ATH35L still remained in the cytoplasm, more than 50% of expressed ATH35L protein for the E. coli signal peptides was secreted into the periplasmic space and the culture medium (Additional file 1: Figure S3). Figure 1 shows the relative levels of ATH35L secreted into the culture medium from the different signal peptides.
