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The added ammonia was assimilated immediately, and no ammonium accumulated in the medium (Figure 4C).
All the selected lines showed proliferation in WPM culture medium (Figure 1).
The growth of all strains, including P. alcaligenes DIP1, on LB complex medium (Figure 3) was very similar.
Indeed, V2FL was detected in cell-conditioned medium (Figure S1H).
The C. jejuni Δppk2 mutant, however, showed no general growth defect in rich medium (Figure S1).
Cytoplasm of disrupted compartments spilled into the surrounding medium (Figure 2).
Glutamine uptake was indirectly measured by assaying residual glutamine in the medium (Figure S1A and B).
This situation was reversed when these cells were transferred to a control medium (Figure S3).
This was also observed when cells were grown in complex medium (LB medium) (Figure 5B).
ER stress/UPR slightly promoted the release of TPD52 in the SKBr3 culture medium (Figure S5E).
Planktonic A. actinomycetemcomitans did not grow in this medium (figure S1).
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CEO of Professional Science Editing for Scientists @ prosciediting.com