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Thus, any analysis about the TSPAN6 expression levels notably its low and medium expression levels in the Tissue 'Pancreas' of the Normal Tissue dataset, must include the amount of contradictions identified in the expressions of TSPAN6.
As shown in Figure S4, there is a very high correlation (R = 0.99) between fragmented/unfragmented sample pairs though there are more outliers at high and medium expression levels than expected for truly identical samples.
A statistical difference was observed in OS for the expression of TWIST1 (p<0.001): the five-year OS for each group was 79% 955% CI, 67% 91%) for those patients with low expression levels; 66.6% (95% CI, 51.9%–81.3%) for patients with medium expression levels; and 40.6% (95% CI, 24.6%–56.6%) for patients with high expression levels (Fig. 1B).
This is also consistent with the contigs of highest expression having slightly lower SNP locus rates than those of medium expression levels (Additional file 11).
We validated that microarrays have acceptable sensitivity and accuracy in detecting differential expression, especially for genes with high and medium expression levels and for detecting > 2-fold changes.
Cluster 1 corresponds to high expression levels, regrouped 17 genes in family A and 19 genes in family P. Cluster 2, with medium expression levels, comprised 19 genes in family A and 18 genes in family P,.
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While PAG-Csk reached a constant medium expression level, cCbl was only weakly and transiently expressed.
21.5% of genes had a medium expression level ranging from 1,000 to 10 FPKM while 78.3% were expressed at low levels of less than 10 FPKM.
In order to have a medium expression level of the transgene, founders with transgene copy number at about 3 5 were used to breed double transgenic mice.
The three copies of MVP1 were all highly similar and had a low to medium expression level.
A medium expression level was observed in the liver, tonsil and kidney tissues, whereas most other porcine tissues exhibited a low pJAB1 transcript level.
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