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While PAG-Csk reached a constant medium expression level, cCbl was only weakly and transiently expressed.
21.5% of genes had a medium expression level ranging from 1,000 to 10 FPKM while 78.3% were expressed at low levels of less than 10 FPKM.
In order to have a medium expression level of the transgene, founders with transgene copy number at about 3 5 were used to breed double transgenic mice.
The three copies of MVP1 were all highly similar and had a low to medium expression level.
However, since this promoter is adjacent to a section of pBR322 that acts as a transcriptional enhancer in yeast, a medium expression level is obtained.
However the transcripts for these copies have a low to medium expression level, which means that high k-mer values are not appropriate.
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Thus, any analysis about the TSPAN6 expression levels notably its low and medium expression levels in the Tissue 'Pancreas' of the Normal Tissue dataset, must include the amount of contradictions identified in the expressions of TSPAN6.
As shown in Figure S4, there is a very high correlation (R = 0.99) between fragmented/unfragmented sample pairs though there are more outliers at high and medium expression levels than expected for truly identical samples.
A statistical difference was observed in OS for the expression of TWIST1 (p<0.001): the five-year OS for each group was 79% 955% CI, 67% 91%) for those patients with low expression levels; 66.6% (95% CI, 51.9%–81.3%) for patients with medium expression levels; and 40.6% (95% CI, 24.6%–56.6%) for patients with high expression levels (Fig. 1B).
This is also consistent with the contigs of highest expression having slightly lower SNP locus rates than those of medium expression levels (Additional file 11).
We validated that microarrays have acceptable sensitivity and accuracy in detecting differential expression, especially for genes with high and medium expression levels and for detecting > 2-fold changes.
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