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As shown in Figure 3B six of the tested genes including flgZ, flhDC, fliC2, PSF113_0118 and PSF113_4631 were clearly regulated by AmrZ under the experimental conditions tested (rich medium, exponential phase).
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The wild type or GE P. putida KT2440 were cultured in the LB medium at exponential phase, and this culture medium (2 mL) was inoculated into flasks containing soil.
Cells were grown in YES medium to exponential phase and imaged on a slide harboring an agarose patch containing YES medium with 3% glucose.
citri cells cultured in either SB or XVM2 medium at exponential phase was isolated using TRIzol® reagent (Invitrogen) according to the manufacturer's instructions.
Extracts were prepared from the wild-type strain and the ΔptsP, ΔptsO and ΔhprK mutants grown in rich medium to exponential phase (OD600 = 0.8).
Yeast strains were grown overnight in SD-Ura medium to exponential phase (OD600 of 0.6 0.8). 100 nM rapamycin was added to the cultures and cells were harvested at various time points.
Strains were grown in YPD medium to exponential phase.
Briefly, fission yeast cells were grown at 25°C in YE5S medium to exponential phase.
Fission yeast cells were grown at 25°C in YE5S medium to exponential phase.
E. coli MC4100 cells were grown at 37°C in rich or minimum medium to exponential phase (OD600nm~0.4), as described [ 55].
An overnight culture was diluted to OD = 0.3 and cells were grown in YPD or YNB medium until exponential phase (4 h) before adding CuSO4 (ProLabo).
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