To investigate whether this phenomenon can be replicated by providing nutrients and removing toxic metabolites for cells cultivated in OptiPro SFM, cell cultivation with medium exchanges was carried out.
No medium exchange was carried out.
Long-term rotation without feeding and medium exchange was also evaluated and was maximized at 40 h.
This all leads to a maximum volume reduction of 10%, as a complete medium exchange was carried out directly after taking the sample at 48 h.
Half-volume medium exchange was performed every 3 days with medium containing fresh cytokines.
In fact for all the data points given in Table 2 a 10-fold medium exchange was necessary to reach a steady state.
For the chase, a quick medium exchange was performed.
The first medium exchange was performed after 48 h to remove nonadherent cells.
After γ-irradiation, cells were left in the incubator for 14 days, during which one medium exchange was performed.
Medium exchange is often used for viral productions despite the fact that this procedure is not easily scalable.
