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No medium exchange was carried out.
Long-term rotation without feeding and medium exchange was also evaluated and was maximized at 40 h.
This all leads to a maximum volume reduction of 10%, as a complete medium exchange was carried out directly after taking the sample at 48 h.
Half-volume medium exchange was performed every 3 days with medium containing fresh cytokines.
In fact for all the data points given in Table 2 a 10-fold medium exchange was necessary to reach a steady state.
Medium exchange was performed twice weekly.
Similar(47)
Medium exchange is often used for viral productions despite the fact that this procedure is not easily scalable.
From these results we concluded that a medium exchange is not required and that CD-U2 supports stable HA virus yields and good TCID50 titers at a CCI of 2 × 10 cells/mL.
To investigate whether this phenomenon can be replicated by providing nutrients and removing toxic metabolites for cells cultivated in OptiPro SFM, cell cultivation with medium exchanges was carried out.
Full medium exchanges were performed twice weekly.
I suppose the development that houses equals medium of exchange isn't all bad.
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