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At pre-determined time points the swollen tablets were taken out of the incubation medium, excess water was removed and the amount of water uptake determined gravimetrically.
EPS production is known to be affected by nutrient status of the growth medium; excess available carbon and limitation of nitrogen, potassium, or phosphate promote EPS synthesis (39).
Thus, compared with patients with a medium excess loss in body mass index (1-50%), the adjusted relative risk was 0.32 (95% confidence interval 0.04 to 2.57) in those with no excess loss in body mass index, and 1.46 (0.55 to 3.85) in those who lost over 50% of their excess body mass index.
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DMSO (200 μL) was added to each well to dissolve completely the internalized purple formazan crystals when the medium and excess MTT were removed.
When OsIRT1 was overexpressed, Cd accumulation in roots and shoots was increased under MS medium containing excess Cd, but this phenotype was not observed in the field condition (Lee and An 2009).
Hajjaj et al. (1997) discovered that Monascus could produce the extracellular red pigments N-glucosylrubropunctamine and N-glucosylmonascorubramine in a chemically defined culture medium with excess glucose and monosodium glutamate (nitrogen source).
Furthermore, in all these new Chelatococcus isolates, a growth-associated accumulation of intracellular PHB granules was observed when growing in medium with excess carbon source (Ibrahim et al. 2010).
To be sure, this caution on the part of households does guard against an over extension that might in time lead to a bust, but it also blocks a rise to that happy medium between excess and greater levels of economic activity.
Steady state expression of FLO11 was measured at different limiting concentrations of ammonium sulphate, when the cells were precultured in the medium containing excess of ammonium sulphate.
Standard SDC medium without excess of essential amino acids was used for comparison.
The early expression of these genes is particularly surprising as the medium contains excess glutamate and is not limited in nitrogen.
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