Sentence examples for medium each triplicate from inspiring English sources

After refreshing infection medium, each triplicate cell well representing different time points (6, 12, 24 and 48 hr) and each bacterial strain was challenged with the infection medium containing bacteria to achieve an infection rate of 1 10.

Briefly, 2 × 10 cells were seeded into 96-well plates in 100  μl of medium in triplicate for each condition.

Cells were cultured for three days in 10 % FCS medium; each treatment was performed in triplicates.

In order to ensure the stability test of each colony, 100 µL was plated on YPD solid medium in triplicate with different concentration of Kan.

For each concentration 50 μL of compound was added to the medium in triplicate.

A volume of 100 µL of crude enzyme and uninoculated control was added to separate 300 µL of CMC containing medium in triplicate while blank sample contained 400 µL buffer.

Three disks (5 mm diameter) per leaf were pooled, ground in sterile water, serially diluted and plated on CPG solid medium in triplicate.

At indicated times post seeding, medium from triplicate wells was removed, cells were washed once with 0.5 ml of 1X phosphate buffered saline (PBS), and trypsinized for 4 minutes at room temperature.

Cells, harvested 48 h post transfection using 5 mM EDTA in PBS, were added (1.25×105 cells/well) in serum free medium to triplicate wells of BD BioCoat™ Matrigel™ Invasion Chambers (BD Bioscience) and complete medium containing 10% FCS was added to the lower chamber.

Doubling dilutions of serum samples collected from the vaccinated animals were made in 100 µl volume of serum-free growth medium in triplicate wells of 96-well plates and incubated with 102 pfu/100 µl of MVANS3 for 2 hours at 37°C, 5% CO2.

Streptomyces strains were cultured in liquid GYM medium in triplicate, as described previously (17).