Sentence examples for medium each time from inspiring English sources

Fifteen mmol/L 3-amino-1,2,4-triazole (3-AT) was added to the growth medium each time to inhibit low levels of His expression in a leaky manner when a potential candidate was validated.

The RA was added fresh in the medium each time. 2 µg genomic DNA were digested with Kpn I (NEB) and treated with sodium bisulfite as described [50].

The chamber was replenished with an equal volume fresh medium each time a sample was withdrawn.

Sink conditions were maintained by replacing an equal volume of release medium each time.

Supernatants were collected at four weekly intervals, replacing the sampled medium each time.

Myelinating cultures were fed three times weekly by replacing one-half of the medium; oligodendrocytes were fed twice-weekly replacing three-quarters of the medium each time.

Every 2 h, the culture medium was changed, and the concentration of HSPB4 in the medium during each time frame was measured by ELISA.

40  μL of 0.5 mg/mL of MTT solution was added with 360  μL growth medium at each time point.

Apolipoprotein B-100 and apolipoprotein B-48 were immunoprecipitated from the medium at each time point using a polyclonal anti-APOB apolipoprotein B antibody (DAKO, Glostrup, Denmark) and Pansorbin cells (Merck).

The data indicated that the ALP activity of cells treated with TGC-CM was significantly higher than that of cells treated with regular medium at each time point (P < 0.001).

The virus-containing medium was exchanged with fresh medium, and at each time point, 50 μl of CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Corporation, Madison, WI, USA) was applied and then incubated at 37°C, 5% CO2 for 2 hours.