Sentence examples for medium during the last from inspiring English sources

When stated, the proteasome inhibitor MG132 (10 µM, Sigma) was added to the medium during the last 16 hours of culture and collected 48 hours postransfection.

Monodansylcadaverine (MDC; 100  μM, Fluka) was added to the medium during the last hour of culture.

To induce serum starvation conditions, hMSCs were cultured in FBS-free medium during the last 24 hours of culture.

To further optimise this process we induced mineralisation in the chondrogenic constructs before implantation by changing to osteogenic medium during the last 7 days of culture.

For cell proliferation assays, 10 μmol/l BrdU (Sigma-Aldrich) was added to the medium during the last hour of culture.

Briefly, the conditioned medium (CM) of ∼40 × 10 cultured cells, maintained in serum-free medium during the last 48 h, was first centrifuged at 300 g for 4 min and then filtered through a 0.22-μm pore-sized filter.

The cells were cultured in iron-free minimal medium during the last 16 h.

Washing medium from the last supernatant was used as control (vehicle).

Although well size was limited during organ development, we attempted to improve the culture environment by changing the medium in the last 80 h.

BrdU (Roche) was added to the incubation medium for the last 6 h of culture.

An extra-dimensional shift (EDS) during the last session rewarded a medium instead of an odor or the reverse.