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Maintaining a medium density of overstorey trees in grazed paddocks can provide both production and biodiversity benefits.
In such cases, selective sweeps may be detectable with a medium density of molecular markers.
Blood plasma was removed and gently added onto 5 mL of Histopaque® lymphocyte separation medium (density of 1.077 g/mL; Sigma-Aldrich, St Louis, MO, USA).
Thus, a threshold of 18 heads/km was considered to be a reasonable cutoff value for the medium density of small ruminants in this study.
These species have been reported to be relatively well adapted to exposed conditions [presence of tank, medium density of trichomes (absorptive scales) and short time-span to maturity (Benzing and Renfrow 1971; Hietz et al. 2002)].
To separate the PBMCs from other blood components, the diluted blood was overlaid onto tubes containing Pancoll separating medium (density of 1.077 g/ml; PAN-Biotech, Aidenbach, Germany) and centrifuged for 25 min at 500 g at room temperature without brake.
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No CA carriers or controls lived in areas with medium densities of non-farrowing swine.
CA carriers and controls had similar odds of living in areas with medium densities of farrowing swine.
Only 1 CA carrier and 0 of their matching controls lived in areas with medium densities of total farrowing swine.
Compared to controls, HA carriers had higher odds of living in areas with medium densities of total (OR: 2.89, 95% CI: 0.30-28.26) and farrowing swine (OR: 2.45, 95% CI: 0.43-13.89), but similar odds of living in areas with medium densities of non-farrowing swine (OR: 0.97, 95% CI: 0.13-7.44).
Adjusted for education, cases had 4.76 times the odds of living in census block groups with medium densities of swine (>0 - 149 swine/square mile), compared to block groups with no swine (95% CI: 1.36-16.69, Table 2).
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