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The analysis of secretomes diluted into culture medium demonstrated that abundant proteins could be relatively quantified within 1.5 20-fold 1.5 20-foldh satisfachangestatistics.
Flask cultivations in a glycerol-based medium demonstrated that the modified strain produced nearly two-fold higher concentrations of 1,3-PD (8.6 vs. 4.75 g/L).
A medium for continuous operation was also designed and, using that medium, demonstrated that cell concentration and interferon-α production increased proportionally according to glucose concentrations in the reservoir during the continuous culture (dilution rate, D = 0.2 1/h).
Culture on this medium demonstrated that sufficient iron is stored intracellularly by N. meningitidis to support one overnight passage of the bacterium, which has important implications for studies of iron restriction.
Analysis of tumour-conditioned medium demonstrated that bexarotene inhibited angiogenic factors and MMPs and increased TIMPs.
Spot dilution growth assays on FK506 medium demonstrated that some CDC228 progeny maintain whereas others lost the FK506 resistance phenotype.
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As shown in Figure 5C, cell viability was significantly improved when H2O2-treated cells were recovered in withanone-supplemented medium demonstrating that whereas MAA caused ROS generation, DNA and mitochondrial damage, withanone protected the cells against such damage by suppressing ROS generation as hypothesized in our model (Figure 2A).
As expected, cdc13Δ TLC1+ and cdc13Δ tlc1Δ mutants were able to grow on YEPD and –URA, but not on FOA medium, demonstrating that CDC13 was essential for survival of these cell types.
The results in protic/co-solvent medium demonstrate that 6CN10 exhibits the characteristics of a nitroaromatic.
Indeed Tortosa and colleagues reported that co-expression of comQ and comX in E. coli leads to the production of active pheromone in the medium, demonstrating that ComQ is the only dedicated protein required for processing, modification, and release of active ComX pheromone (Tortosa et al. 2001).
Experiments carried out in a bubble-column fixed-bed reactor in the presence of distilled water as a reaction medium, demonstrate that nitrates can be efficiently removed from the liquid-phase, and that the maximum contaminant level for ammonium ions in drinking water is not exceeded.
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