Sentence examples for medium deficient in from inspiring English sources

The Δpxa1 strain was transformed with the pxa2::his using PEG-LiAc method (Gietz and Schiestl [2007]) to construct double deletion strain Δpxa1&2.Transformed deletion stains Δpxa1&2 strain were selected via histidin prototroph by growing on synthetic complete minimal medium deficient in histidine.

For stable isotope labeling by amino acids in cell culture (SILAC)[19] RPMI1640 medium deficient in Arg and Lys was used (Pierce® SILAC quantification kit, Thermo Fisher Scientific, Bonn, Germany) and supplemented with 10% dialyzed FBS, 0.1 g/l [12/13C6]L-lysine·2HCl, 0.2 g/l [12/13C6]L-arginine·HCl (Cambridge Isotope Laboratories, Inc., Andover, USA), and 2 mM L-glutamine (Invitrogen).

The response of most of these genes is faster in the presence of the V-ATPase inhibitors than it is to medium lacking LDL, perhaps because medium lacking LDL does not block the delivery of cholesterol from LDL particles that are either bound to their receptor at the cell surface or already released into the endocytic pathway at time the medium deficient in LDL was added.

Protein interaction tests were assessed on triple dropout medium deficient in Trp, Leu, and His or quadruple dropout medium deficient in Trp, Leu, His, and adenine.

The transformed yeast cells were selected on synthetic minimal double dropout medium deficient in Trp and Leu.

A medium deficient in nitrogen lead to the accumulation of storage carbohydrates such as trehalose and glycogen and structural modifications.

Cells were grown in cell culture medium deficient for L-arginine and L-lysine (SILAC RPMI, Thermo Fisher Scientific), supplemented with 2 m M L-glutamine (Gibco, Invitrogen), 10% dialyzed fetal bovine serum (Invitrogen) and amino acids of different molecular weight (0.389 m M L-arginine and 0.789 m M L-lysine; Sigma Aldrich) for at least five doublings.

Mutants impaired in cysteine biosynthesis entering the stationary phase display a glycogen-excess phenotype when they are cultured in the Kornberg medium, which is ascribed to stringent response-mediated up-regulation of glg genes occurring when the culture medium is deficient in cysteine precursors.

Whereas values of YxATP considerably larger than YgrowthxATP are obtained whenever the medium is deficient in any of the necessary growth substrates it is surprising that values of YxATP much smaller than YgrowthxATP can also be obtained, namely for growth on yeast extract at conditions where the growth reactions are neither limited by the carbon-, the nitrogen- or the energy source.

The feed medium was deficient in a nitrogen source.

For in vitro studies we used the human SKMEL-188 melanoma line that is amelanotic when cultured in F-10 medium relatively deficient in melanin precursors, but produces melanin pigment when cultured in a mixture of DMEM and F-10 (75 : 25) that contains an increased concentration of melanin precursors including -tyrosine (Slominski et al, 1999).