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CSCs isolated from U343MG cells were plated in NSC medium (day 0) and treated for 7 days with 100 nM CHA, 50 nM BAY606583, and 100 μM TMZ, alone or in combination (until day 7).
After 18 days of culture in osteogenic medium (day 40 from withdrawal), the cells showed 70 75% confluence, and the presence of aggregates or nodules of calcium mineralization was appreciable.
During the first 2 weeks, the initially high (mean) RWR of the biofilms (day 5 41.4 ± 9.8) decreased to a medium (day 7 19.6 ± 6.0) and virtually low level (day 14 10.7 ± 4.5).
Briefly, myoblasts grown in light proliferation medium (day 0) were divided into two batches, one expanded in heavy medium and the other in light medium.
For differentiation experiments, cells were plated at a concentration of 4×10 cells/cm in the indicated differentiation medium (day 0).
Cells were grown to near confluency in their corresponding complete medium (day 0) and then were trypsinized and seeded at lower density in the absence of LIF for 1 day (day 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com