In aqueous medium, CR shows an absorption band at 498 nm and 350 nm (n → π *), transition associated with the azo group [23].
DPC were found at elevated levels in lymphocytes in several human populations with low to medium Cr exposures.
Specifically, the amount of p53 present in cells cultured in medium with CR serum was 1.6-fold lower than that of the same cells cultured in medium with AL serum (Fig. 7A, right panel).
We therefore initiated our CR studies in normal human fibroblasts such as WI-38, MRC-5 and IMR-90 cells, by limiting the concentration of their main caloric resource, glucose, in cell culture medium to obtain CR.
The wear test was performed on medium size Columbus CR TKA (Aesculap AG Tuttlingen, Germany) with the thinnest available gliding surface.
Four groups of data were compared: FxCR 8 mJ with ADSC-CM (T1), FxCR 8 mJ with DMEM medium (C1), Fx CR 16 mJ with ADSC-CM (T2), and FxCR 16 mJ with DMEM medium (C2).
In this study, we found an elevation in superoxide level during CA, which was more clearly seen for standard growth conditions (2% dextrose in YNB medium) than under CR conditions (0.5% dextrose in YNB medium).
Target cells (NPA cell line) were removed from culture flasks using HEPES EDTA buffer, rinsed and aliquots (∼10 cells) incubated (37°C, 60 min, 5% CO2) in culture medium 100 μCi Cr.
When low passage IMR-90 cells (PDL 37) infected with Ad-SIRT1-siRNA were subsequently exposed to medium containing 10% CR rat serum for 72 hours, their growth rate was improved (i.e. they attained higher cellular density) compared to that displayed by the same cells treated with medium containing 10%% AL serum Fig. 6E).
Target cells were used to determine spontaneous Cr released in culture medium only and total Cr release was assessed by addition of HCl (1 N).
