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When the extracts of soybean curd residue were added to 24-h pre-cultured nutrient-broth medium (corresponding to 1 g wet soybean curd residue per mL medium), antibiotic production in the liquid medium was clearly improved in the culture (Additional file 1: Figure S1).
Free CUR, free CUR dissolved with the aid of DMSO and CUR@PVP nanofiber, with various concentrations of CUR in medium (corresponding to 5, 10, 20, or 40 μg/ml CUR), were subsequently prepared and added to the 96-well plates.
To test the accuracy of our new 2-D code for regions including coastlines, we considered the case where a cylinder full of a conductive (4 S/m) medium corresponding to seawater is embedded in a resistive (100 ohm.m) whole-space.
In this paper, two analytical impulse responses in a uniformly moving medium, corresponding to two cases that the flow direction is parallel to and perpendicular to the hologram plane, are derived first with consideration of flow effects, and then RT-NAH is extended to realize forward and backward propagation of time-dependent signals in the moving medium.
LDH activity in the medium, corresponding to macrophage necrosis, was measured as an absorbance at 490 nm using an ELISA plate reader (SpectraMax 250; Molecular Devices).
The infected cell culture was then replenished by the appropriate growth medium (corresponding to the differentiation state of the NHBE cells).
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Here, we consider two classes: medium, corresponding, respectively to degrees 1 to 4, and high, corresponding to degrees 5 and above.
Results showed that a voltage of 0.54 V was produced using Se-free medium solution, corresponding to an anodic power density of 2900 mW/m2.
A culture medium sample corresponding to 1.5×106 cells was run in polyacrylamide gel electrophoresis and transferred to an Immobilon-P Transfer Membrane (Millipore).
Subsequently, the mixture of 100 µl (for A549 or HEK293) or 200 µl (for HeLa) protein/BioPORTER-complex and medium (typically corresponding to a total amount of 100 ng TrxR or SecTRAP) was added to each well, containing cells that had first been washed with serum-free medium.
Dip at syllable onset is particularly pronounced over medium frequencies corresponding to formants.
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