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Negative (containing only plant cell suspension culture) and positive (compound 1 in the medium) controls were also prepared.
Corresponding PBS/DMSO aliquots for medium controls were also prepared.
Positive (2.4 mM, EMS) and negative (F-12 medium) controls were included in each experiment.
Positive (CVS-27 and EBLV-1) and negative (cell culture medium) controls were incorporated in each test.
To test whether these cells migrate in a FCS-directed manner, FCS-free medium controls were included for each cell line.
Similar(55)
In addition, untreated cell controls, cell-free treatments and a culture medium control were included for each extract.
Similar dilutions of individual drugs and the drug-free medium control were included in each test plate.
Values were multiplied by their corresponding dilution factor, background from un-stimulated medium control were subtracted from each antigen response both for IFN-γ and IL-17; the cytokine concentration was expressed in pg/ml.
P value comparing Bps, SA, and PMA to medium control was determined via unpaired t test after log transformation of the data.
Appropriate control (medium control) was employed.
Natural cell death (medium control) was 22%, of which 5% was caused by apoptosis and 17% was caused by necrosis.
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