Sentence examples for medium control wells from inspiring English sources

Responses were determined by subtracting the SFU values from medium control wells from the peptide induced response.

Net cytokine responses were calculated by subtracting the cytokine concentrations in the medium control wells from those in supernatants derived form samples stimulated with TLR agonists.

Assuming the number of stimuli to be tested is not large it may be necessary to include separate medium control wells for each cell number used as background spot counts may also be affected.

A similar background in the medium control wells was observed for all three conditions.

To increase the power to detect differences between spot counts in experimental versus medium control wells, we therefore recommend using six medium control wells for each sample, whenever possible.

Two wells were filled with soluble or LD antigen while the rest were filled with the medium (control wells).

The precursor frequency was calculated as [(number of spots in experimental well - number of spots in medium control well)/total number of cells per well] ×106.

Incubating the MEG-01 cells for 48 h with 10 or 100 ng ml−1 IL-6 yielded mean VEGF-values of 5.34±1.6 pg ml 1−1 and 4.72±0.6 pg ml 1−1×10 cells, which were not significantly higher than the VEGF concentration in the conditioned medium of control wells.

Data were normalized to DNA content, determined using supplied protocols (CyQUANT-NF, Invitrogen; 50 µl prepared reagent per well, including medium-only control wells).

Data were normalized to DNA content, determined using supplied protocols (CyQUANT-NF, Invitrogen; 50 μl of prepared reagent per well, including medium-only control wells).

Migration index was calculated by dividing the number of migrated cells by the mean migration seen in the medium-only control wells.