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Exact(5)
P value comparing Bps, SA, and PMA to medium control was determined via unpaired t test after log transformation of the data.
Appropriate control (medium control) was employed.
Natural cell death (medium control) was 22%, of which 5% was caused by apoptosis and 17% was caused by necrosis.
The cells were washed once with PBS to remove any cell debris, and either fresh medium containing various concentrations of the metal complexes or fresh medium (control) was added to each well.
Cell culture medium containing 1000 U/ml recombinant IFN-α or normal growth medium (control) was used to replace growth medium of cells 16 hr prior to infection with the indicated viruses (MOI = 0.03).
Similar(55)
In addition, untreated cell controls, cell-free treatments and a culture medium control were included for each extract.
Similar dilutions of individual drugs and the drug-free medium control were included in each test plate.
Values were multiplied by their corresponding dilution factor, background from un-stimulated medium control were subtracted from each antigen response both for IFN-γ and IL-17; the cytokine concentration was expressed in pg/ml.
Negative (containing only plant cell suspension culture) and positive (compound 1 in the medium) controls were also prepared.
Corresponding PBS/DMSO aliquots for medium controls were also prepared.
Positive (2.4 mM, EMS) and negative (F-12 medium) controls were included in each experiment.
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