Sentence examples for medium control for from inspiring English sources
Cells were then treated with free celecoxib (IC50), celecoxib-loaded liposomes (IC50), and empty liposomes (equivalent to volume liposomal celecoxib) and culture medium (control) for 48 h.
Cells were treated with ENP stocks: 5 mg/ml and 1 mg/ml, 0.5 mg/ml, 0.1 mg/ml, 10 μg/ml, 1 μg/ml of the TiO2, SiO2, and CeO2 (Sigma-Aldrich, MO, USA) or L1 medium (control) for 48 h.
After washing 3 times, the cells were re-stimulated with α-CD3, α-CD3/α-CD28, or medium control for 24 hr and cytokines in the supernatant were measured by ELISA.
Cells (2 × 10) plated in glass coverslips were grown for 24 h and then incubated with Pt or 0.1% DMSO M199 medium (control) for 24 h.
We incubated PBMCs from untreated CLL patients with fludarabine (1 μg/ml), mafosfamide (1 μg/ml) or DMSO (medium control) for 24 h.
Thereafter, cells were incubated with 100 μl/well LPS (Fc 1 μg ml−1) or culture medium (control) for 20 h for PGE2 and cytokine production or with 100 μl/well ConA (Fc 3 μg ml−1) or culture medium (control) for 44 h both for proliferation and cytokine production.
For library tests, blank wells with medium (with or without chemicals) were used as medium controls for data processing.
Fourth, a region of the design space was identified where fresh weight growth was increased 198% and 67% over the MS medium controls for nonembryogenic and embryogenic tissues.
To test whether cells migrate in an FCS-directed manner, we included FCS-free medium controls for each of the tested cell lines, without noting cell migration in any case (data not shown).
Three different A549 cultures were exposed to five concentrations of SiO2 NPs (0.1, 1.0, 1.5, 3.0, and 6.0 μg/cm) and serum-free medium (control cells) for 24 h, and to two concentrations (3.0 and 6.0 μg/cm) for 72 h.
Assuming the number of stimuli to be tested is not large it may be necessary to include separate medium control wells for each cell number used as background spot counts may also be affected.
