Vehicle-treated conditioned medium (control conditioned medium, CCM) or PGF2α-treated conditioned medium (PCM) was then centrifuged and the supernatant frozen at −20 C until use.
Nevertheless, while most of the studies evaluated solvent and medium control conditions, the investigation of process blanks in bioassays was reported only by a small number of studies.
After eclosion, 0- to 2-d-old adult flies were transferred to vials containing either standard corn meal/molasses medium (control conditions) or 1% agarose in water (starvation conditions).
One well was cultured for 21 days on expansion medium as control condition for the histochemical stainings.
Alternatively, utilizing four wells for both, medium control and experimental conditions, would give similar power to detect differences [ 21].
HEK293T, HCT116 and H1299 cells were serum starved for 24 h before treatment with either control condition medium (EV-CM), Wnt3a conditioned medium (Wnt-CM) for another 24 h.
The transcriptome analysis clarified distinctive metabolic pathways under three different growth conditions, static culture, high temperature, and xylose medium, in comparison to the control condition of glucose medium under a shaking condition at 30°C.
However, when GFP-PIST-transfected OK cells were adapted acutely (for two hours) by incubating them to either low (0.1 mM) or to high (2 mM) Pi culture medium starting from the control condition, 1 mM Pi, the classical adaptation was blunted by the PIST overexpression.
Sputum samples from CF individuals infected with the same B. cenocepacia strain as genome isolate were used, hence, other than a dilution into a minimal growth medium (used as the control condition), no further treatment of the sputum was carried out.
