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Basic ratio was 2.7±0.66 (mean±SD) in medium control and moderate higher in LPS stimulated cells (3.5±0.92), while the ratio was significant higher after 48 h of palmitate stimulation (5.7±1.1).
Response determination is made based on a comparison of the medium control and experimental wells.
Alternatively, utilizing four wells for both, medium control and experimental conditions, would give similar power to detect differences [ 21].
Each mutant colony was inoculated onto two sister plates: one containing just Bristol medium (control) and the other containing diphenylamine (25 µM).
When reverted to full medium, control and Sec24CD-depleted cells started to proliferate again, whereas the number of Sec24AB-depleted cells continues to diminish.
The RNA was collected from pancreatic PANC-1 cells grown in a serum-rich medium ("control") and 24 h following the removal of the serum ("treatment").
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Hep-2 cells and stromal fibroblasts were seeded at a density of 1 × 106 cells/mL per 75 cultureture flasks in complete medium (controls) and in conditioned medium.
These values were normalised to the non-conditioned medium controls and expressed as a percentage of the total TGFβ in non-strained controls.
All data were corrected for various controls, including blank with medium control (with and without water samples) and promoterless bacterial controls (with and without water samples).
M. tuberculosis colony forming units (CFUs) were enumerated in the drug-free medium (control tubes) and in the tubes with anti-tuberculosis drugs.
The % inhibition was calculated by (RLA in pseudotypes and medium control – RLA in pseudotypes and immune serum in a given dilution)/RLA in pseudotypes and medium control.
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