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This yield was comparable to the costly conventional synthetic medium containing yeast extract, urea, soybean oil and glucose.
Debaryomyces nepalensis NCYC 3413 was maintained on a solid YEPP medium containing yeast extract 10 g/l, peptone 20 g/l and pectin 5 g/l at pH 7.0 and incubated at 30°C for 26 h and later stored at 4°C.
SSF experiments were performed in 100 mL bottle containing 100 g/L pretreated rice straw suspension in 25 mL medium containing yeast extract (Oriental yeast, Tokyo, Japan), 5 g/L; (NH4 2SO4, 7.5 g/L; KH2PO4, 3.5 g/L; MgSO4·7H2O, 0.75 g/L; CaCl2·2H2O, 1.0 g/L (Wako Pure Chemical Industries, Ltd. Osaka, Japan) and the pH was adjusted at 5.5.
Two unidirectional cDNA libraries from A. euteiches were prepared from mycelium grown on medium containing yeast extract/glucose (library MYC) and from mycelium in interaction with Medicago truncatula roots (library INT) [20].
Δ gpr-11 mutants grew better than wild type in medium containing yeast extract (Table 2).
Notably, the effect was greatly diminished in medium containing yeast extract, so a defined medium was used in this experiment.
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The YPD medium contained yeast extract of 5 g/L, peptone of 20 g/L, and dextrose of 20 g/L.
The basal medium contained yeast extract 15 g/L, KCl 0.5 g/L, MgSO4·7H2O 0.5 g/L, K2HPO4 5 g/L, NaNO3 2 g/L.
YX medium contained yeast extract (10 g/L) and xylose (58 g/L).
YPD medium contained yeast extract (10 g/L), peptone (from casein, 20 g/L) and glucose (20 g/L).
At OD600 nm 0.4 the culture was split, and one half was supplemented with 1 mM cAMP (LB medium contains yeast extract and will contain trace amounts of cAMP, e.g. see Polnisch and Hofmann) [ 14].
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