Sentence examples for medium containing viruses from inspiring English sources

Filtered medium containing viruses carrying PDGFRβ was used to infect PDGFRα+/+ β−/− MEF.

Briefly, female flies 3 5 days old were anesthetized on ice and injected intra-abdominally with ∼100 nl of Dulbecco's modified Eagle medium containing virus at the selected concentration.

Medium containing virus was removed from cells and replaced by 2% complete medium.

For transduction, targeted cells were incubated with medium containing virus for 24 h.

Medium containing virus was collected following 24 and 48 h post transfection and filtered through 0.45-μm filters.

Culture medium containing virus was harvested every 24 h for 72 h, and centrifuged 10,000 × g overnight at 4 °C.

To transduce ASCs, conditioned medium containing virus with dsRed and neomycin resistance was added to ASCs at 70%% confluence.

K562, HL60 and Jurkat cells were incubated with medium containing virus supplemented with 8 μg/ml polybrene for 24 h.

After an 18 hour-incubation, all culture medium containing virus was removed and cultures were further incubated with fresh medium for up to 6 days.

The filtered medium containing virus particles was then added to the target transduction cells (H1975), which were plated the day before transduction.

On the day of harvest, cell culture medium containing virus was collected and centrifuged for 10 minutes at 525 × g and 4°C to remove cell debris.