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Clonal recombinant cultures were established by colony purification on a solid complex medium containing tryptone (0.2% w/v).
The E. coli cells were routinely cultivated in Luria-Bertani (LB) medium (containing tryptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L).
Shake flask cultivations were carried out using LB medium containing tryptone (10 g L-1), yeast extract (5 g L-1), sodium chloride (10 g L-1), and ampicillin (100 μg mL-1), at pH 7.
For co-culture experiments, a 1 1 mixture of the two strains was grown in Brock's medium, containing tryptone (0.2%, w/v) and glucose (0.2%, w/v), at 75°C.
Frozen bacterial strains were streaked for single colonies and one single colony was used for inoculation of liquid TY medium containing tryptone (Hi-Media, Mumbai, India) 8 g/L, yeast extract (Hi-Media) 5 g/L, and sodium chloride 5 g/L.
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Luria Bertani (LB) medium contained tryptone (10 g), yeast extract (5 g), and NaCl (10 g).
Terrific broth (TB) medium contained tryptone (12 g), yeast extract (24 g), K2HPO4 (72 mM), KH2PO4 (17 mM), and glycerol (4 mL).
Shaking flask cultivations SK1 and SK 3 were performed in medium (3) containing tryptone instead of casamino acids, but 0.2 g IDA-1 beads were applied in SK1 while triazine beads were used in SK3.
S. acidocaldarius DSM 639 and S. solfataricus DSM 1617 cultures were grown at 79°C in modified Allen [ 46] mineral base medium containing 0.2% tryptone.
Briefly, parallel lines of phage λKH54 and λi21c were drawn on plates containing tryptone broth (TB) medium, TB +0.2% glucose, or TB +0.2% arabinose and allowed to soak into the plate.
Escherichia coli strains were grown at 37 or 30 °C on a Luria Bertani (LB) medium (Becton, Dickinson and Company, NJ, USA) containing tryptone (10 g/L), yeast extract (5 g/L) and NaCl (5 g/L), and 100 µg/ml kanamycin and 100 µg/ml ampicillin were added to the medium when necessary.
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