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Induction + glass (I + GLASS): Kairomone medium containing the glass piece.
Control + glass (C + GLASS): Pure medium containing the glass piece.
Control + HDPE (C + HDPE): Pure medium containing the piece of HDPE.
Induction + PET (I + PET): Kairomone medium containing the piece of PET.
Induction + HDPE (I + HDPE): Kairomone medium containing the piece of HDPE.
Control + PET (C + PET): Pure medium containing the piece of PET.
A non-physiologically relevant medium containing the cationic surfactant, cetrimide, was also investigated.
Next cells were incubated with fresh medium (phenol red-free) or medium containing the pro-oxidant, dimethoxy-naphthoquinone (DMNQ, 10 μM) (Sigma-Aldrich).
After 6 h, the medium containing the transfection reagent was removed and replaced with fresh DMEM containing 10% fetal bovine serum and 1% P/S.
Briefly, 5-d old seedlings grown vertically were transferred to fresh medium containing the indicated compound (Sigma-Aldrich) at the specified concentration.
The medium containing the secreted protein was collected after 3 4 days of incubation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com