Sentence examples for medium containing sorbitol from inspiring English sources

Subsequently MIC of an antimicrobial is increased several fold in the test medium containing sorbitol.

In short, pre-cultures of T. reesei Rut-C30 were first cultivated on a minimal medium containing sorbitol as a carbon source.

Previously, we demonstrated that addition of osmoremedial growth medium containing sorbitol reduced the actin requirement during yeast endocytosis, suggesting actin polymerization was required to generate sufficient inward force to invaginate the membrane against the outward force of turgor pressure [ 26].

Then the cells were concentrated by centrifugation for 5 minutes at 800 × g, re-suspended in minimal medium containing 1 M sorbitol and 0.8% agar and poured into petri-dishes.

However, the AS fraction from the gas1Δ mutant grown in medium containing 1.2 M sorbitol had a lower amount of the high molecular weight β(1,3 -glucans, as compared to the wild-type strain grown under the same conditions.

Spheroplasts were permeabilized with 0.2 mg/ml nystatin at 30°C in respiration medium containing 1 M sorbitol, 0.5 mM EGTA, 2 mM MgSO4, 1.7 mM NaCl, 10 mM potassium phosphate (pH 6.8), 0.1% BSA.

The cells were loaded in a microfluidic chip and the medium was exchanged within seconds by medium containing 0.5 M sorbitol.

Three-hundred microliters were spread to YPD medium containing 1 M sorbitol and incubated at 24° for 4 hr, and biolistic transformation was then performed as described previously.

For the hypo-osmotic stress experiment (Fig.  6e,f), cells were grown overnight in SD medium containing 1 M sorbitol, diluted in the morning in the same medium to OD600 0.05, and grown for at least 4 hours before the start of the experiment.

Fermentors (2 L) containing sorbitol medium (900 mL) were then inoculated with the precultures and grown in batches for 24 h (30 °C, 750 rpm stirrer speed, aeration with 1 L min−1 air) before being switched to continuous mode (pH maintained at 5.0).

The growth was examined after 2 days for YPD control medium, or 6 to 10 days for plates containing sorbitol or ethanol.