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Induction (I): Kairomone medium, containing solely kairomone as a positive control for the induction.
Therefore, both strains were pre-grown in Mandels-Andreotti (MA) medium containing solely glycerol as carbon source.
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The control medium contained only 0.5% ethanol.
Ionic liquids are liquids containing solely ions having melting points lower than 100 °C.
Formulations containing solely one active drug should be preferred.
Each plate contained four negative controls: two medium blanks (solely Schneider's medium and Schneider's medium containing larvae suspension reflecting non-induced and induced conditions), and two fluorescence blanks (P. luminescens pBR-cherry harbouring the promoter-less mcherry reporter), under non-induced and induced conditions.
Control + glass (C + GLASS): Pure medium containing the glass piece.
Induction + glass (I + GLASS): Kairomone medium containing the glass piece.
On the next day, culture medium was replaced with neurobasal medium containing B27 supplements and AraC.
For the clonogenicity assay, medium was replaced with fresh medium containing CK-666 every three days.
After 24 h, the medium containing viral particles was replaced with fresh medium.
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