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Further, co-culturing rat MSC with isolated rat liver cells in medium containing several growth factors induced expression of hepatocyte-specific genes [9].
After incubation, the inoculum was discarded, and cells were replenished with RPMI-1640 growth medium containing several concentrations (0, 0.1, 0.2, 0.3, 0.4, and 0.5 mM) of ribavirin.
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Immunoblot analysis revealed that the skin cell line medium contained several activin and bone morphogenetic protein moieties.
ASC conditioned medium contains several growth factors including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and may mimic the beneficial effects of cells [ 10, 11].
β-Glucosidase production by Debaryomyces pseudopolymorphus UCLM-NS7A using a simple nutrient medium containing cellobiose was evaluated under several biochemical and physiological parameters in submerged fermentation.
We found that embryonal rhabdomyosarcoma (eRMS) cell lines can form rhabdomyosarcoma spheres (short rhabdospheres) in stem cell medium containing defined growth factors over several passages.
Transfected cells were selected and cloned in a medium containing neomycin (250 μg/ml) for several weeks before clonal dilution.
The induced roots were subcultered several times on medium containing decreasing concentrations of cefotaxime to get the bacteria free hairy root cultures lines.
Transferring and adapting the strains several times to the medium containing perchlorate and lactate or methanol did not result in a substantial reduction of perchlorate to chloride.
Subsequently MIC of antimicrobial is increased several fold in the test medium containing such compounds.
Subsequently MIC of an antimicrobial is increased several fold in the test medium containing sorbitol.
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