Sentence examples for medium containing serial from inspiring English sources

Medium was removed and replaced with 200 µL of complete medium containing serial two-fold dilutions of freshly harvested supernatants from 100 ng p24/mL.

The cells in each well were incubated with 100-μL complete medium containing serial concentrations of Au NR@SiO2 nanoparticles (0~36 culture. After 24 h of incubation, 10 μL sterile CCK-8 per well was added and continued to incubate for 2 h at 37 °C (5%% CO2).

Briefly, 5 × 10 cells per well were seeded in 96-well plates and treated for either 48 h with medium containing serial concentrations of carboplatin (0 60 μg/mL) or 72 h with medium containing serial concentrations of paclitaxel (0 100 ng/mL).

Each kinase was incubated in a reaction medium containing serial dilutions of NS-018, biotinylated peptide substrate, ATP and MgCl2 in a streptavidin-coated plate for 1 h at 30 °C.

A monolayer of fibroblasts in individual wells of 96-well plates was incubated for 3 days with growth medium containing serial, 2-fold dilutions of butein ranging from 10 μg/mL to 1.25 μg/mL.

This was determined by incubation in growth medium containing serial dilutions of compounds in the range of 50 – 2000 µg/mL for 2 days, after which the viable cell numbers were determined.

The next day, fresh medium was replaced containing serial diluted CDIM9 between 1 × 10-3 mol/l and 1.9 × 10-6 mol/l and cultured at 37°C for 48 hours.

The next day, the medium was replaced with fresh medium with FBS (10%) containing serial dilutions of the samples (3.9 – 1000 μg/mL) previously sterilized in a 0.45 μm membrane.

Then we replaced the medium with another 100 μL of growth medium that contained serial diluents of our compounds and then incubated the cells at 37 °C and 5% CO2 for an additional 72 h.

Medium was then replaced with fresh RPMI containing serial dilutions of viral supernatant.

Cells from overnight cultures were diluted 100-fold with fresh medium, grown to OD600~0.3, and 10  μL aliquots were inoculated into 96-well plates containing serial dilutions of the particular toxicant in 150  μL of antibiotic-amended medium and plates were incubated for 48 h at 37°C with gentle shaking.