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To evaluate the effects of gefitinib and/or meta-iodobenzylguanidine, cells were incubated with either control medium or a medium containing rising concentrations of the respective drug or drug combination.
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In particular, serial subcultures of C. albicans ATCC 10231 were performed in fresh medium every 48 hours, containing rising concentrations of M.
The presence of dimethyldithiocarbamate (Me2DTC) in a medium containing ammonia (borax-NH4Cl and NH4OH-NH4Cl buffer) and cobalt II) gives rise to a catalytic hydrogen wave.
Preincubation with a medium containing no Ca2+ ions (10 mM ETGA, 0 M Ca2+) almost abolished the intracellular rise in [Ca2+] induced by 10−7 M ET (Fig. 3D).
When cultured in N2 medium containing FGF-2 and EGF under free-floating conditions, colonies of undifferentiated hES cells gave rise to floating spheres.
Control + glass (C + GLASS): Pure medium containing the glass piece.
Induction + glass (I + GLASS): Kairomone medium containing the glass piece.
For the clonogenicity assay, medium was replaced with fresh medium containing CK-666 every three days.
After 24 h, the medium containing viral particles was replaced with fresh medium.
On the following day, the medium was renewed with HepaRG medium containing 2% dimethyl sulfoxide.
On the next day, culture medium was replaced with neurobasal medium containing B27 supplements and AraC.
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