Sentence examples for medium containing recombinant from inspiring English sources

Medium containing recombinant virus was recovered two days after transfection and 10 ml of virus-containing medium was used to transduce 2.5×106 Hcells cells for 24 h.

The culture medium containing recombinant virus was harvested, filtered and stored at 4°C.

To assess the effect of a diffuse source of reelin, MAM treated slices were incubated in medium containing recombinant reelin (1 nM) or plain medium as a control.

Splenocytes were infused with CFSE, cultured for 24 h with agonists, washed with fresh medium, and re-cultured for 72 h with medium containing recombinant murine IL-2 (rmIL-2 [Peprotech; Rocky Hill, NJ]).

For culturing cells as spheres we have used the mammosphere assay based upon culturing neural stem cells in serum-free medium containing recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (rhbFGF) [11], [19].

Furthermore, EPC-CM supplemented with 1 µg/ml of anti-PDGFR β antibody (α-PDGFRβ, AF385, R&D Systems, UK), and control medium containing recombinant human PDGF-BB (rhPDGF-BB) in a final concentration of 100 pg/ml and 100 ng/ml, respectively, were compared.

After formation of control and mutant organoids, fresh medium was replaced containing recombinant mouse IL-6 (100 ng/ml), IL-6 neutralizing antibody (1 μg/ml), BAY11-0782 (10 μM), or no supplement.

Strips of this membrane were incubated either with conditioned medium containing the recombinant C. elegans agrin fragment or with purified recombinant chicken agrin fragments representing the muscle (A0B0) and neuronal (A4B8) agrin isoforms.

After 6 hours, the media was changed, and the medium containing the recombinant viruses was collected at 48 and 72 hours post transfection for two independent infections.

Cells were then stimulated in fresh FBS-free medium containing human recombinant IL-1β (10 ng/ml), TNF-α (10 ng/ml), IL-4 (10 ng/ml), IgE (1 or 10 µg/ml) or vehicle (medium alone) for time periods specific to experiments, as mentioned below.

The medium containing the recombinant proteins was centrifuged to remove any cellular debris.