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Cells on cover glass were washed and mounted with mounting medium containing propidium iodine (Vectashield).
Finally cells were mounted by mounting medium containing propidium iodide (PI) (Vector Laboratories).
The supernatant of each well was then discarded and 150 µL of culture medium containing propidium iodine (PI, FL-3) at 50 µg/mL was added.
After a 5-min wash and a 20-min incubation at 37°C with RNAse A 0.5 mg/ml in PBS, slides were mounted with mounting medium containing propidium iodide (Vectashield, Vector Laboratories, Inc., Burlingame, CA) and sealed with nail polish.
The slides were stained with Vectashield mounting medium containing propidium iodide and analyzed with an Olympus BX51 light microscope.
The cells were harvested, resuspended in medium containing propidium iodide (7.5 μM) and analyzed by flow cytometry.
Similar(49)
The cells were washed, harvested, resuspended in medium containing Propidium Iodide (7.5 µM) and analyzed by flow cytometry.
After PBS washes, the cells were incubated with Cy2-labeled secondary antibody (1 250; Jackson ImmunoResearch, Newmarket, UK), washed in PBS, air dried and coverslipped with fluorescence mounting medium (Vector, Burlingame, CA, USA) containing propidium iodide.
After washing, cells were incubated in binding buffer containing propidium iodide (PI).
The T cells were resuspended in the FACS buffer containing propidium iodide (PI) and immediately analyzed by flow cytometry.
Alternatively, the T cells were resuspended in the FACS buffer containing propidium iodide (PI) and immediately analyzed.
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