Sentence examples for medium containing polybrene from inspiring English sources

After letting the virus adsorb for 30 60 min, the medium containing polybrene was added back to the cells.

Following incubation, medium containing polybrene was removed and replaced with normal growth medium (NGM), and the cells incubated for an additional 24 hrs under normal cell culture conditions before taking post-transfection photographs of cells.

To infect cells, HEK293 were trypsinized, resuspended in the medium containing polybrene (10 µg/ml; Sigma), and allowed to attach to the dish at a confluence of 60 80%.

Prior to infection, the lentivirus-containing media were thawed completely at room temperature, and mixed with the same amount of fresh medium containing polybrene (8 μg/ml).

In a checkerboard titration manner, tumor cells (1 × 10) were cultured in different sets of T-75 flasks in complete growth medium containing polybrene (Sigma Chemicals, St . Louis MO) (0.5 ~ 16 μg/ml) and β- Gal vector (1 ~ 20 cfu/cell) at different concentrations.

The lentiviruses were diluted in 0.2 mL complete medium containing polybrene (8 mg/mL) and added to the cells for 12 h of incubation at 37°C, followed by incubation in 0.3 mL of freshly prepared polybrene-DMEM for 24 h.

This supernatant was filtered through a 0.22 um filter, then replaced the cell growth medium of Hela by 5 ml viral supernatant, containing polybrene (8 μg/ml) to help the adhesion of viruses.

Cells were transduced for 16 h with viral supernatants containing polybrene (8 mg/ml), washed in PBS and cultured in complete medium containing puromycin (2.5  μg/ml) obtained from Invivogen (Invivogen, Toulouse, France).

Medium on established NIH 3T3 L1 cultures was replaced with medium containing 8 μg/mL polybrene.

To infect N/TERT keratinocytes, NHEK and SK-UT-1B cells were pre-incubated for 10 min at 37 °C in normal growth medium containing 5  μg/ml polybrene (Sigma-Aldrich), before replacing with retroviral supernatant containing the same concentration of polybrene (Sigma-Aldrich).

Cells were pre-incubated for 5 minutes in growth medium containing 5 µg/ml polybrene (hexadimethrine bromide; Sigma) before replacing with retroviral supernatant containing the same concentration of polybrene to facilitate infection.