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Enhanced enzyme activity and gene expression were observed in both glucose-containing medium and medium containing only cellulose.
If the nutritional requirements of a microorganism are known, a chemically defined medium containing only those chemicals can be prepared.
The aim of this work was to optimize the glycerol:ornithine molar ratio in the feed medium containing only these compounds to maximize CA production in continuous cultivation.
In contrast to the culture medium containing 5% FBS supplemented with F-12 used in that study, we used medium containing only 2% FBS in order to imitate the tendon microenvironment, which lacks blood circulation.
When agricultural wastes of coba husk and corn steep liquor were used in the ratio of 4.5 0.5, a 22.5% increase of xylanase activity was observed compared with the medium containing only coba husk.
At the end of the 6-day fermentation period, the medium containing only animal fat (5%) and corn steep liquor (2.5%) yielded the maximal reduction in surface tension (from 50 to 28 mN/m).
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The seed culture medium contained only low concentration of xylose from the pretreated corn stover feedstock and no glucose or pre-hydrolysate in it at the beginning of the culture.
The control medium contained only 0.5% ethanol.
Spent medium contained only the intact fusion proteins; there was no indication of degradation.
The cells failed to proliferate and even showed a decrease in cell number when the culture medium contained only galactose (Additional file 2: Figure S1D, F).
Absent the nitrogen contributed by watermelon juice amino acids, the second fermentation medium contained only nitrogen contributed primarily by the molasses.
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