Sentence examples for medium containing normalized from inspiring English sources

3T3-L1 (ATCC, VA) preadipocytes (passages 4 10) were preconditioned for 3 days in either α-MEM culture medium (Control) or α-MEM medium containing normalized TSF.

Ten pools were constructed with each pool containing normalized DNA of 6 bulls of equimolar amounts.

After 4 min incubation, the medium containing EGTA was washed three times with normal medium.

The signals were imaged on the Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA) and then normalized by internal controls, and the values for cytokines in clear medium containing 10% FBS were background subtracted.

Briefly, epithelial cells (3×104/35 mm dish) were seeded in growth medium containing 1.375 mM CaCl2 at least 2 d prior to incubation with CM from PRE or SEN cells, normalized for equal cell number per ml.

After wash, cells were cultured for an additional 70 h in fresh medium containing Chk1 inhibitors under normoxia (21% O2), and the viable cells were quantified using either AlamarBlue or ATP assay and normalized using either vehicle for single treatment or Chk1 inhibitor alone for combination treatment.

Control + glass (C + GLASS): Pure medium containing the glass piece.

Induction + glass (I + GLASS): Kairomone medium containing the glass piece.

On the next day, culture medium was replaced with neurobasal medium containing B27 supplements and AraC.

For the clonogenicity assay, medium was replaced with fresh medium containing CK-666 every three days.

After 24 h, the medium containing viral particles was replaced with fresh medium.