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Isolated ventricular myocytes were cultured overnight in medium containing normal glucose (n=5.5 mM) or high glucose (HG=25.5 mM).
Hemichannel activity under oxidative stress (induced by CSE and H2O2) was examined by LY uptake from the extracellular medium containing normal Ca++.
Culture of S. japonicum for 24 h with 20% anti-LD1+ anti-LD2 anti-sera resulted in a 65% reduction in glucose (P≤0.0001) compared with the level in worms cultured in medium containing normal serum (Figure 4C).
When cells are grown in medium containing normal sulphate supplementation, as performed here, sulphation of heparan sulphate is selectively inhibited, with 6-O-sulphation inhibited before 2-O-sulphation [64].
For high glucose treatment cells were cultured for 1 hour with medium containing 30 mM D-glucose or with medium containing normal glucose concentration supplemented with Mannitol as osmotic control.
No alterations were observed in the cells cultured in the medium containing normal magnesium levels.
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Human hepatoma HepG2 cells (ATCC HB 8065, ATCC, VA, USA) were maintained in Dulbecco's modified eagle medium (DMEM) containing normal glucose (5 mM glucose), supplemented with 10% fetal bovine serum (FBS), and 100 U/mL penicillin (GIBCO, Aukland, NZ) in an incubator (37°C and 5% CO2).
These cells were then transferred to a medium containing the normal isotope of nitrogen, 14N, and allowed to go through cell division.
Calli were then transferred for 1 day to fresh ENT post-thawing 2 medium supplemented with 0.5 M sucrose, after which they were transferred again to ENT recovery medium containing a normal sucrose concentration (234 mM) and 1 mM CaCl2.
Astrocytes (1×105) were seeded in each well of a 6-well plate containing normal medium.
6 hours after siRNA transfection, medium was changed to normal medium containing half the normal concentration of Penicillin-Streptomycin. Nocodazole was used at 0.5 µM in DMSO.
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