Sentence examples for medium containing neomycin from inspiring English sources

HAT resistant clones were tested in a medium containing neomycin and puromycin.

Transfected cells were selected and cloned in a medium containing neomycin (250 μg/ml) for several weeks before clonal dilution.

Four tagged transformants (#s 32, 33, 34 and 35) were plated on L-DOPA medium containing neomycin at 10X the inhibitory concentration (10 × 106 cells per plate).

A single colony of each tagged strain was grown in YPD overnight at 30°C and then plated on L-DOPA medium containing neomycin (2 mg/ml).

Twenty-four hours after transfection, the cells were transferred to selection medium containing neomycin (G418 500 μg/mL) for three weeks.

For recombinant protein production, transfected CHO cells were expanded to near confluence in selective medium containing neomycin, and then were grown for 3 4 days in serum-free medium.

Additional medium was then added to a total of 12 ml, the cells were allowed to grow for 48 h, harvested by trypsinization and then distributed into 96-well plates containing neomycin (0.5 mg/ml) selection medium.

Seeds from plants dipped with constructs containing neomycin phosphotransferase II (NPTII) were grown on ½ Murashige and Skoog medium (MS) plates [ 64] with 50 μg/ml kanamycin, 0.6% (w/v) agar and 1% (w/v) sucrose to obtain transgenic plants with kanamycin resistance.

The GLO I PCR product was digested with HindIII and cloned in the vector pGEM-7zfαNeoα (containing neomycin phosphotransferase as the selection marker) to create pGEM-7zfαNeoα-LdGLOI containing the neomycin phosphotransferase gene.

A GFP-tagged Kb vector containing neomycin selected-marker was provided by Dr. Edidin [42] (Department of Biology, Johns Hopkins University, Baltimore, Maryland).

For example, the prfA G145S mutant is neomycin-resistant because of the actA-gus-neo-plcB transcriptional fusion it contains, but the WT camR strain is neomycin-sensitive, so aliquots from mixed cultures of the prfA G145S and WT camR strains were also plated on BHI agar containing neomycin.