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The culture medium was then replaced by 100 μl of serum-free DMEM medium containing nanoparticles (100 600 mg/mL).
The cells were incubated for 24 h before replacing the medium with medium containing nanoparticles or 20 ng/ml free Nile Red.
After 24 h of culture, the medium in the wells was replaced with the fresh medium containing nanoparticles in the concentration range of 0 123.52 μg/ml.
After 24 h incubation, the medium containing nanoparticles was removed from the plate to make sure that no nanoparticles remain in the solution and avoid overlap or hinder MTT assay.
After another 18 h, the medium containing nanoparticles was removed.
After completion of exposure time, the culture medium containing nanoparticles was removed and cells were harvested using 0.25% trypsin-EDTA.
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The medium was replaced with medium containing either nanoparticles or free Nile Red, or the dual-labelled nanoparticles.
UV-vis spectrum of the aqueous medium containing gold nanoparticles showed a peak around 560 nm.
UV vis spectrum of the aqueous medium containing gold nanoparticles showed a peak at around 540 nm.
UV-vis spectra of the aqueous medium containing silver nanoparticles showed a surface plasmon resonance peak at 418 nm.
Open image in new window Fig. 7 Comet assay showing DNA damage in sperms of Hydroides elegans incubated in medium containing silver nanoparticles.
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