For a trypan blue staining, we diluted 1 part of the 0.4% prepared trypan blue to 9 parts of medium, which contained multiple myeloma cells, then mixed them and incubated them for 5 minutes.
The choice of cell culture medium becomes increasingly relevant, with complex cultures containing multiple cell types where media for one cell type may influence the phenotype of the co-cultured cell population.
Prepare a 35 mm tissue culture dish containing multiple droplets of embryo culture medium, and a 4-well plate with each well containing 500 μl of embryo culture medium.
Neoplastic cells had ample eosinophilic to finely vacuolated cytoplasm and medium-sized nuclei to very large and anaplastic nuclei containing multiple large eosinophilic nucleoli with multinucleated cells occasionally evident.
c Containing multiple transmembrane helices.
Pressure waves, identical to each other both in frequency and magnitude, generated by acoustic devices in mutually opposite directions through a viscous medium form standing waves contained multiple nodes and anti-nodes.
Biological tissue is a complex medium, as it often contains multiple layers with miscellaneous structures having different scattering and absorption coefficients.
Nitrilase of N. globerula NHB-2 was hyperinduced through multiple feeding of isobutyronitrile in the growth medium containing 1.0% glucose,0.5% peptone, 0.3% beef extract, and 0.1 % yeast extract (pH 7.5) described previously ([Sharma et al. 2011]).
The acclimatization of this yeast strain was conducted by multiple rounds of the adaptive culture in inhibitors medium containing acetic acid, furfural, and phenol (Sinopharm Chemical Reagent Co.,Ltd, Shanghai, China).
To minimize possible phenotypic changes in gCSCs due to prolonged maintenance in vitro and multiple passages, early passage gCSCs were frozen in neurosphere medium containing 5% DMSO and stored at −70°C.
