Sentence examples for medium containing mouse from inspiring English sources
Neurospheres were plated onto poly-d-lysine coated coverslips in NeuroCult™ NSC basal medium containing mouse NeuroCult™ NSC proliferation supplements and 2% fetal calf serum (Sigma-Aldrich) without growth factors.
TGF-β3 neutralizer added to the medium containing mouse serum inhibited the effect.
Forty-eight hours after transfection, the cells were incubated in cold DMEM medium containing mouse M1 anti-FLAG antibody at 2 µg·mL–1 and incubated 1 h at 4°C.
To further investigate the significance of pancIl17d for embryonic development, we plated the surviving pancIl17d knockdown blastocysts in medium containing mouse LIF and inhibitors for MEK1/2 (MandK1/2) and GSK3β (2i medium), conditions that are frequently utilized for the culture of ground-state ESCs, and harvested the cultures after 10 days.
For T98G cells the plating efficiencies were 0.48 ± 0.07 for cells receiving serum from unirradiated mice and 0.48 ± 0.06 for cells receiving serum from irradiated mice. 2 μg/ml TGF-β3 neutralizer (AF243-NA, R&D systems, Minneapolis, MN, USA) was added to the medium containing mouse serum before transfer to reporter cells.
Unless otherwise mentioned, all experiments were conducted in medium containing DHS. Mouse peritoneal macrophages were isolated by a standard procedure from WT C57Bl6/j and SphK-1 KO mice.
We performed serial tenfold dilutions in cell culture medium containing healthy mouse brain homogenate.
H2Kd+HLA+ cells were grown in CFU medium containing either mouse recombinant growth factors or human recombinant growth factors for 18 days.
For the cholesterol efflux, medium containing 2.5% mouse plasma from the 4-weeks and the 8-weeks studies was added to cells.
Zona-free 4-cell embryos were cultured in 500 ng/mL Activin A until E4.5 and then seeded on feeder cells containing mouse ESCs medium plus PD0325901 and CHIR99021 (2i) (Fig. 4A).
Following differentiation, cells were stimulated with 50 µg/ml CuOx-LDL in the presence or absence of 10 µg/ml of HDL in full culture medium containing 1% mouse serum for 24 h to induce foam-cell formation.
