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The modification of the initial medium composition was performed every three days, where an aliquot of the previous culture medium (10%) was added as inoculum to a new culture medium containing more phenol.
In case of medium containing more than 6.5% ethanol, the SGR of ethanol-adapted strain became larger than that in non-adapted strain.
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The most consistent effluent was obtained using the smaller medium since, at flow rates above 0.4 l min−1, the BAF using the larger medium produced an effluent containing more than 20 mg l−1 of suspended solids for over 30 min after backwashing.
Southern blot analysis of DNA isolated from DBY746 cells at this time point confirmed that cells cultured in medium containing 0.5% glucose contained more mitochondrial DNA relative to nuclear DNA compared to cells cultured in medium containing 2% glucose (Fig. 2H).
The Ca peak present in original NAu-1 has been replaced by the K peak, suggesting cation exchange with the aqueous medium which contains more K than Ca (see Table II).
An additional culture where cells were grown in a richer growth medium (containing 10 times more pyruvate, oxaloacetate and taurine) was used for the same assay performed on cells during logarithmic growth.
The resistant sublines were maintained in the medium containing CDDP for more than 10 months.
In most cases, apart from sarcomatoid RS5 cells, those grown in medium containing DFBS were more sensitive to pemetrexed than cells grown in medium containing normal FBS, with the largest difference observed in JL1 and DM3 cell lines.
Pellets cultured in conditioned medium contained significantly more GAGs than those cultured in expansion medium and 3(d)).
Although a larger medium volume initially contains more oxygen, nutrients and space for growth of bacteria, the void in the container and subsequently oxygenation of the medium will be decreased.
It has been reported that stationary phase cells [ 16, 24, 48] and cell grown in complex medium containing yeast extract are more difficult to disrupt [ 16].
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