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To this end, strains were spotted and grown on MOKA medium containing milk proteins.
If no ammonium is present, wild type strains produce a halo, due to extracellular protease activity, on medium containing milk as a nitrogen source.
Extracellular protease activity is low on medium containing milk as a carbon source, as carbon starvation is required to stimulate extracellular protease production when ammonium is present.
The ndtAΔ mutant also displays a halo but the xprG2 mutant, which is protease-deficient, does not when grown on medium containing milk as a nitrogen source.
Like the xprG2 single mutant, the xprG2 ndtAΔ double mutant produced no halo on medium containing milk as a carbon or nitrogen source and did not undergo autolysis in response to nutrient stress.
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A culture medium containing formula milk was designed, which allowed a balanced growth of intestinal microorganisms and was used to perform fecal batch cultures from preterm babies.
The swab was transported in a medium containing skim milk, tryptone, glucose, and glycerol.
When tested on solid medium containing skim milk, the phenotype of the imeBΔ xprGΔ veA + and imeBΔ xprGΔ veA1 strains were indistinguishable both were protease-deficient.
To each of the NPS specimens, 800 μl of normal saline (NS) was added for processing; then, 140 μl of this fluid was transferred to a medium containing skim milk, tryptone, glucose, and glycerine (STGG media).
Optimization strategy was conducted to enhance the enzyme production by S. aureus in a medium containing a waste material (milk whey).
Besides, LAB were screened for proteolytic activity and EPS production on MRS medium containing sucrose and skim milk, respectively.
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